Wednesday, 08 May 2024 11:54

Berlin: Liver tissue model produced without materials of animal origin Featured

As reported by Technische Universität Berlin, scientists at TU Berlin have succeeded in producing a model of the liver from human cells using 3D bioprinting without having to resort to materials of any animal origin. Up to now, in vitro research has often relied on fetal calf serum (FCS) as culture medium and laminins or collagens from mice.

The production and use of "chimera" tissue is now a thing of the past: Ahmed Ali, a doctoral student in Prof. Jens Kurreck's working group, has developed a chemically precisely defined culture medium consisting of growth factors, insulin, selenium, sugars and salts to cultivate human liver cells. The cells, which have been acquired commercially, must first be weaned from the FCS in which they are supplied. The second challenge was then to adapt the new medium precisely to the needs of human liver cells. To replace the murine collagen (Matrigel), a human collagen was isolated from human placentas.

The functionality of the cells, from which a two- and three-dimensionally printed human liver metabolic tissue has been printed, has already been demonstrated: The human liver cells react sensitively to cytotoxic substances such as okadaic acid, which is produced by algae.

Original publication:
Ali, A.S.M.; Berg, J.; Roehrs, V.;Wu, D.; Hackethal, J.; Braeuning, A.;Woelken, L.; Rauh, C.; Kurreck, J. (2024).Xeno-Free 3D Bioprinted Liver Model for Hepatotoxicity Assessment.Int. J. Mol.Sci. 2024, 25, 1811.

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