Thursday, 22 August 2013 13:13

Genome of the Chinese hamster decoded Featured

Under the direction of Prof. Dr. Alfred Pühler from the Centre for Biotechnology (CeBiTec), Bielefeld University, a research team has decoded the genome of the Chinese hamster.

Also involved in the research were scientists from the University of Natural Resources and Life Sciences in Vienna led by Prof. Dr. Nicole Borth, the Austrian Centre of Industrial Biotechnology (ACIB) as well as the pharmaceutical companies Novartis (Switzerland) and Pfizer (USA).

First the chromosomes of the hamster strain 17A/GY were separated using a flow cytometer. Then the individual chromosomes were sequenced separately with the next-generation technology. With the help of modern equipment for high-throughput sequencing, billions of short DNA sequences were produced. Using a further method, these short sequences were assembled to individual chromosomes.

The results are of great importance for research groups working with hamster cell lines.

Compared to the older Sanger sequencing, the NGS method offers diverse benefits, such as personnel, time and financial savings. Researchers expect that any individual's DNA could be sequenced at an affordable price in the foreseeable future. This method is of particular importance in research for personalised medicine.

Original publication:
Karina Brinkrolf, Oliver Rupp, Holger Laux, Florian Kollin, Wolfgang Ernst, Burkhard Linke, Rudolf Kofler, Sandrine Romand, Friedemann Hesse, Wolfgang E. búdách, Sybille Galosy, Dethardt Müller, Thomas Noll, Johannes Wienberg, Thomas Jostock, Mark Leonard, John Grillari, Andreas Tauch, Alexander Goes, Bernhard Helk, John E. Mott, Alfred Pühler and Nicole Borth: Chinese hamster genome sequenced from sorted chromosomes, Nature Biotechnology,