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Biologielaborantin
Zurzeit befinde ich mich im 4. Lehrjahr meiner Ausbildung am Lehrstuhl für Zell- und Entwicklungsbiologie der Universität Würzburg, welche ich Ende Februar 2012 abschließen werde. Dort führe ich verschiedene molekularbiologische Analysen von Proteinen aus, z. B. mit Westernblots, 2D-Gelelektrophoresen und indirekten Immunfluoreszenzen. Zudem habe ich Methoden der DNA-Klonierung sowie Arbeiten in der Zellkultur und Elektronenmikroskopie kennengelernt. Ich bin ein verantwortungsbewusster und zuverlässiger Mensch und kann selbstständig sowie auch im Team arbeiten. Die mir übertragenen Aufgaben führe ich sorgfältig aus. Darüber hinaus bin ich gerne bereit, neue Arbeitsbereiche kennenzulernen (z. B. mikrobiologische Prüfungen oder Botanik) und mich weiterzubilden. Ich habe zudem fundierte Kenntnisse im Bereich der EDV.
Promotion
Ich habe mein Biologie Studium im November letzten Jahres erfolgreich abgeschlossen und bin auf der Suche nach einer Promotionsstelle für die ich mich begeistern kann. Ich möchte zukünftig an Tierversuch Ersatz im Sinne der 3R forschen. In meiner Diplomarbeit habe ich an neurodegenerativen Erkrankungen geforscht und mir neben molekularbiologischen in-vitro Methoden auch Fähigkeiten im bioinformatischen Bereich angeeignet. Was den Promotionsbeginn angeht bin ich flexibel, da ich derzeit eine Stelle als wissenschaftliche Mitarbeiterin habe.

"...In all its complexity the animal is a black-box..."


About the animal protection research award of Rhineland-Palatinate at 30th june 2011

For the third time, the state Rhineland-Palatine has awarded the price for the promotion of research into replacement and supplement to animal experiments. This year the expert jury has voted for the research group´s works of Prof. Dr. Claus-Michael Lehr, Dr. Eva-Maria Collnot and Fransisca Leonard from the University of the Saar, Department Pharmacy, Biopharmacy and Pharmaceutical Technology and from the Helmholtz Institute for Pharmaceutial Research Saarland (HIPS), Department Drug Delivery.



On 30th of June the three price winners got awarded for their project “„A three-dimensional coculture of enterocytes, monocytes and dendritic cells to model inflamed intestinal mucosa in vitro”. It deal with the development of an in vitro-coculture system consists of an enterocyte cell line (CaCo-2) and the generation of a process of intestinal inflammation for the study of alterations of the cell barriere properties after addition of for instance pharmaceuticals.

With an introduction the office manager of the environment ministry of Rhineland-Palatinate, Dr. Erwin Manz informed that animal protection is a major concern of the state government. Animal experiments should get more reduced, especially by the funding of alternative methods. In Rhineland-Palatinate, the current numbers of laboratory animals were stagnating. At this time, 117,144 animals were used in this state, for this reason, alternatives have to be found and their application has to be accelerated. The price should be "...an advocate to strengthen research on this field". Futhermore, it was said, as Rhineland-Palatinate "...was a small contry, that applications for the price from other contrie`s researchers are allowed.


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Dr. Erwin Manz, office manager of the Environment Ministry of Rhineland-Palatinate.
Photo: Christiane Hohensee



In her speech, Dr. Christiane Baumgartl-Simons from the regional Association People for Animal Rights Rhineland-Palatinate and jury member praised the scientists whose "...set out to develope animal-free methods, since in vitro-methods have the potential for elegant, powerful and ethical unencumbered scientific methods which also exclude  - in the opposite to animal models - species-caused differences in the pathogenesis." By this means scientists with ambitions and visions were rare in the current research landscape.

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Left image: Dr. Baumgartl-Simons, Dr. Erwin Manz, Mrs. Müller-Taschinski, Mr. Manfred Braun (Head of Public Relations of Helmholtz Centre for Infection Research) and Prof. Dr. Claus-Michael Lehr.
Right image: Prof. Dr. Claus-Michael Lehr.

Photo: Christiane Hohensee



In his acceptance speech Prof. Claus-Michael Lehr said the research group were glad in the price. The award were a motivation s that the method will find the way into free economy as quick as possible. At the same time he praised his team: "The reason for such a result is a good functionating team". Little pressure caused by the animal protection law to search for alternatives is also a stimulus for the science. "In all its complexity the animal is a black-box", a reduction and a simplification is reasonable if it comes to biological barriers. In the opposite to an animal experiment with a new developed method one cannot only say THAT it works, but HOW it works.

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From left to right: Manfred Braun, Fransisca Leonard, Prof. Dr. Claus-Michael Lehr, Dr. Eva-Maria Collnot, Dr. Christiane Baumgartl-Simons and Dr. Christiane Hohensee.
Photo: Mr. Hirsch, Environment Ministry Rhineland-Palatinate.